Debottlenecking mevalonate pathway for antimalarial drug precursor amorphadiene biosynthesis in Yarrowia lipolytica
نویسندگان
چکیده
منابع مشابه
The non-mevalonate pathway of isoprenoid precursor biosynthesis.
The recently discovered non-mevalonate biosynthetic route to isoprenoid precursors is an essential metabolic pathway in plants, apicomplexan parasites, and many species of bacteria. The pathway relies on eight enzymes exploiting different cofactors and metal ions. Structural and mechanistic data now exist for most components of the pathway though there remain some gaps in our knowledge. The ind...
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Carotenoids are a class of molecules with commercial value as food and feed additives with nutraceutical properties. Shifting carotenoid synthesis from petrochemical-based precursors to bioproduction from sugars and other biorenewable carbon sources promises to improve process sustainability and economics. In this work, we engineered the oleaginous yeast Yarrowia lipolytica to produce the carot...
متن کاملYarrowia lipolytica in Biotechnological Applications
The nonconventional yeast Yarrowia lipolytica has been developed as a versatile and attractive tool for a large variety of biotechnological applications. This yeast has several physiological properties with industrial significance. Y. lipolytica uses hydrophobic substrates such as n-alkanes, oils, fats, and fatty acids as low-cost carbon sources. The yeast is able to produce a set of diverse ad...
متن کاملEngineering Yarrowia lipolytica for Campesterol Overproduction
Campesterol is an important precursor for many sterol drugs, e.g. progesterone and hydrocortisone. In order to produce campesterol in Yarrowia lipolytica, C-22 desaturase encoding gene ERG5 was disrupted and the heterologous 7-dehydrocholesterol reductase (DHCR7) encoding gene was constitutively expressed. The codon-optimized DHCR7 from Rallus norvegicus, Oryza saliva and Xenapus laevis were ex...
متن کاملA molecular genetic toolbox for Yarrowia lipolytica
BACKGROUND Yarrowia lipolytica is an ascomycete yeast used in biotechnological research for its abilities to secrete high concentrations of proteins and accumulate lipids. Genetic tools have been made in a variety of backgrounds with varying similarity to a comprehensively sequenced strain. RESULTS We have developed a set of genetic and molecular tools in order to expand capabilities of Y. li...
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ژورنال
عنوان ژورنال: Metabolic Engineering Communications
سال: 2020
ISSN: 2214-0301
DOI: 10.1016/j.mec.2019.e00121